Interleukin-1beta expression and phospholipase A(2) activation after intestinal ischemia/reperfusion injury.
Identifieur interne : 002158 ( Main/Exploration ); précédent : 002157; suivant : 002159Interleukin-1beta expression and phospholipase A(2) activation after intestinal ischemia/reperfusion injury.
Auteurs : Guang-Tao Yan [Niger] ; Xiu-Hua Hao ; Hui Xue ; Lu-Huan Wang ; Ying-Li Li ; Li-Ping ShiSource :
- Sheng li xue bao : [Acta physiologica Sinica] [ 0371-0874 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : ARN messager, Interleukine-1, Phospholipases A.
- métabolisme : Interleukine-1, Ischémie, Lésion d'ischémie-reperfusion, Phospholipases A.
- Animaux, Expression des gènes, Femelle, Intestins, Mâle, Rat Wistar, Rats.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Interleukin-1, Phospholipases A, RNA, Messenger.
- chemical , metabolism : Interleukin-1, Phospholipases A.
- blood supply : Intestines.
- metabolism : Ischemia, Reperfusion Injury.
- Animals, Female, Gene Expression, Male, Rats, Rats, Wistar.
Abstract
The experiments were carried out to explore the interactions between IL-1 beta gene expression, protein level and phospholipase A(2) PLA(2) inhibition after intestinal ischemia/reperfusion injury. Using a rat intestinal ischemia/reperfusion injury model, after collecting the serum, lung lavage, abdomen cavity lavage and important organ tissue samples from control, injury and PLA(2) inhibitor treated groups, IL-1 beta level was measured by radioimmunoassay, and the mRNA expression of IL-1 beta and type II PLA (2)was determined by RT-PCR. After 6 h of injury, the IL-1 beta level in serum was significantly higher than that in the control group; an increase in IL-1 beta was also observed in abdomen cavity lavage 1 or 3 h after injury. IL-1 beta was significantly increased in liver tissue after injury, but was not changed obviously in the lung, kidney and intestinal tissues. IL-1 beta in the lung lavage was significantly higher than that of control group. The mRNA expression of IL-1 beta in lung tissue was increased after injury, but type II PLA(2) mRNA expression was decreased. There were different changes in IL-1 beta level and gene expression after treatment with PLA(2) inhibitor chloroquine, cyclo-oxidase inhibitor indomethacin, or PAF receptor antagonist SR27417 respectively after injury. All these results indicate that after intestinal ischemia/reperfusion injury, the IL-1 beta level and mRNA gene expression are significantly increased, however, the relationship among IL-1 beta, PLA(2) activation and its metabolite release remains to be further elucidated.
PubMed: 11930237
Affiliations:
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of PLA, Beijing 100853</wicri:regionArea><wicri:noRegion>Beijing 100853</wicri:noRegion></affiliation></author><author><name sortKey="Hao, Xiu Hua" sort="Hao, Xiu Hua" uniqKey="Hao X" first="Xiu-Hua" last="Hao">Xiu-Hua Hao</name></author><author><name sortKey="Xue, Hui" sort="Xue, Hui" uniqKey="Xue H" first="Hui" last="Xue">Hui Xue</name></author><author><name sortKey="Wang, Lu Huan" sort="Wang, Lu Huan" uniqKey="Wang L" first="Lu-Huan" last="Wang">Lu-Huan Wang</name></author><author><name sortKey="Li, Ying Li" sort="Li, Ying Li" uniqKey="Li Y" first="Ying-Li" last="Li">Ying-Li Li</name></author><author><name sortKey="Shi, Li Ping" sort="Shi, Li Ping" uniqKey="Shi L" first="Li-Ping" last="Shi">Li-Ping Shi</name></author></analytic><series><title level="j">Sheng li xue bao : [Acta physiologica Sinica]</title><idno type="ISSN">0371-0874</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Expression</term><term>Interleukin-1 (biosynthesis)</term><term>Interleukin-1 (metabolism)</term><term>Intestines (blood supply)</term><term>Ischemia (metabolism)</term><term>Male</term><term>Phospholipases A (biosynthesis)</term><term>Phospholipases A (metabolism)</term><term>RNA, Messenger (biosynthesis)</term><term>Rats</term><term>Rats, Wistar</term><term>Reperfusion Injury (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (biosynthèse)</term><term>Animaux</term><term>Expression des gènes</term><term>Femelle</term><term>Interleukine-1 (biosynthèse)</term><term>Interleukine-1 (métabolisme)</term><term>Intestins ()</term><term>Ischémie (métabolisme)</term><term>Lésion d'ischémie-reperfusion (métabolisme)</term><term>Mâle</term><term>Phospholipases A (biosynthèse)</term><term>Phospholipases A (métabolisme)</term><term>Rat Wistar</term><term>Rats</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interleukin-1</term><term>Phospholipases A</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interleukin-1</term><term>Phospholipases A</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term><term>Interleukine-1</term><term>Phospholipases A</term></keywords><keywords scheme="MESH" qualifier="blood supply" xml:lang="en"><term>Intestines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ischemia</term><term>Reperfusion Injury</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interleukine-1</term><term>Ischémie</term><term>Lésion d'ischémie-reperfusion</term><term>Phospholipases A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Expression</term><term>Male</term><term>Rats</term><term>Rats, Wistar</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Femelle</term><term>Intestins</term><term>Mâle</term><term>Rat Wistar</term><term>Rats</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The experiments were carried out to explore the interactions between IL-1 beta gene expression, protein level and phospholipase A(2) PLA(2) inhibition after intestinal ischemia/reperfusion injury. Using a rat intestinal ischemia/reperfusion injury model, after collecting the serum, lung lavage, abdomen cavity lavage and important organ tissue samples from control, injury and PLA(2) inhibitor treated groups, IL-1 beta level was measured by radioimmunoassay, and the mRNA expression of IL-1 beta and type II PLA (2)was determined by RT-PCR. After 6 h of injury, the IL-1 beta level in serum was significantly higher than that in the control group; an increase in IL-1 beta was also observed in abdomen cavity lavage 1 or 3 h after injury. IL-1 beta was significantly increased in liver tissue after injury, but was not changed obviously in the lung, kidney and intestinal tissues. IL-1 beta in the lung lavage was significantly higher than that of control group. The mRNA expression of IL-1 beta in lung tissue was increased after injury, but type II PLA(2) mRNA expression was decreased. There were different changes in IL-1 beta level and gene expression after treatment with PLA(2) inhibitor chloroquine, cyclo-oxidase inhibitor indomethacin, or PAF receptor antagonist SR27417 respectively after injury. All these results indicate that after intestinal ischemia/reperfusion injury, the IL-1 beta level and mRNA gene expression are significantly increased, however, the relationship among IL-1 beta, PLA(2) activation and its metabolite release remains to be further elucidated.</div></front></TEI><affiliations><list><country><li>Niger</li></country></list><tree><noCountry><name sortKey="Hao, Xiu Hua" sort="Hao, Xiu Hua" uniqKey="Hao X" first="Xiu-Hua" last="Hao">Xiu-Hua Hao</name><name sortKey="Li, Ying Li" sort="Li, Ying Li" uniqKey="Li Y" first="Ying-Li" last="Li">Ying-Li Li</name><name sortKey="Shi, Li Ping" sort="Shi, Li Ping" uniqKey="Shi L" first="Li-Ping" last="Shi">Li-Ping Shi</name><name sortKey="Wang, Lu Huan" sort="Wang, Lu Huan" uniqKey="Wang L" first="Lu-Huan" last="Wang">Lu-Huan Wang</name><name sortKey="Xue, Hui" sort="Xue, Hui" uniqKey="Xue H" first="Hui" last="Xue">Hui Xue</name></noCountry><country name="Niger"><noRegion><name sortKey="Yan, Guang Tao" sort="Yan, Guang Tao" uniqKey="Yan G" first="Guang-Tao" last="Yan">Guang-Tao Yan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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